Influence of putrescine and thidiazuron on in vitro organogenesis in Salvia officinalis L.

In this study, an indirect organogenesis regeneration protocol for Salvia officinalis L. is described. Leaf (0.5 × 0.5 cm) and internode (1 cm in length) explants sections, from 45 day old in vitro germinated plant, were cultured in Murashige and Skoog (MS) basal medium supplemented with 12 different combinations of 2,4-dichlorophenoxy acetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). The best calli induction response was observed when 1 mg/l NAA and 0.5 mg/l BAP combination was applied to the internode explants. Also, results showed an interesting possibility for the stimulation of shoot regeneration (5.66) through internode segments cultured on MS medium containing 50 mg/l putrescine and 0.5 mg/l TDZ. Shoot elongation and rooting were achieved on MS medium with 1mg/l IBA. Excellent acclimatization to greenhouse conditions was observed for all transferred plantlets. By this procedure no morphological differences were observed between the regenerated and mother plants. This protocol may be also utilized to carry out plant regeneration after genetic transformation, in order to develop transformed plants without the presence of chimeric zones.