Response of Estrogen-related Receptor Alpha (ERRα) to Endurance Training and its Participation in Endurance Training-induced Adaptations in Lipid Metabolism in Skeletal Muscle of Male Wistar rats

The aim of the present study was to investigate the expression of estrogen-related receptor alpha (ERRα) - an important factor in cellular energy homeostasis regulation - in skeletal muscle of male Wistar rats after four weeks of endurance training and to determine its role with fat metabolism indexes.
In this experimental study, 30 male Wistar rats (eight weeks-old) were randomly divided into four groups: control (n=7), control尤ﯷ (n=8), endurance training (n=8), and endurance training尤ﯷ (n=7). ERRα was inhibited by intraperitoneal injection of XCT790 (0.48 mg/kg.day) on daily bases. Four weeks of endurance training (five times per week) started at 15 m/min for 20 min and reached to 27 m/min for 50 min was performed by the animals from trained groups. Expression of ERRα, Medium-chain acyl-CoA dehydrogenase (MCAD) and Carnitine Palmitoyl Transferase 1 (CPT-1β) mRNA was measured by Real-Time PCR and quantified by method. One-way analyses of variance were used for cross-group comparison.
The expression of ERRα (P˂0.037), MCAD (P˂0.001), and CPT1β (P˂0.001) mRNA in endurance training group was significantly higher than the control group. The expression of MCAD (P˂0.001) and CPT1β mRNA (P˂0.001) in the endurance training尤ﯷ group was significantly lower compared to those values of the endurance training group.
In sum, expression of ERRα is a trainable factor and its changes are parallel with the increase in expression of lipid metabolism indexes; so, it could have a direct role in endurance training-induced adaptation in fat metabolism.