The effect of alcoholic extract of Panicum miliaceum L. seed on hippocampus neuronal density in male mouse

Hippocampus organization is a part of temporal lobe, which consists of several sections including hippocampal body, dentate gyrus and subiculum. Panicum miliaceum L. contains proteins, vitamins and antioxidants for human health. This study was conducted to examine the effect of the alcoholic extract of the seed of Panicum miliaceum L. plant on hippocampus neuronal density.
In this experimental study, 24 male mice were divided into 4 groups (n=6, each group). The alcoholic extract of the seed of the Panicum miliaceum L. plant was prepared by soxhlet extraction. Three doses of the extract 25, 50, 75 mg/kg were intraperitoneally injected to 3 treatment groups for 21 days and the control group received normal saline injection. At the end of the experiment, the animals were anesthetized and after perfusion, their brains were removed from the skull. After tissue processing, slices of the brain were prepared and stained. Then, different regions of the hippocampus were photographed and neuronal densities were evaluated.
Results showed that the neuronal density in the CA1, CA3 regions of the group treated with 50 mg/kg of the alcoholic extract and in all regions of hippocampus (CA1,CA2,CA3) in groups treated with dose of 75 mg/kg of the alcoholic extract had a significant increase compared to the control group (P The present study shows that the alcoholic extract of the seed of Panicum miliaceum L. plant increases neuronal density and induces neurogenesis in the mouse hippocampus.