Effect of extraction methods on the antioxidant properties of Pimpinella affinis

The purpose of this study was to investigate the effect of different extracting conditions on antioxidant and antimicrobial activity of pimpinella affinis. Samples were extracted by using maceration, ultrasonic assisted method (UAE) and supercritical fluid extraction (SFE). Extraction yield and total phenolic compounds (TPC) of extracts were measured and the antioxidant activity of were analyzed in terms of four concentration (500, 1000, 1500 and 2000 ppm) using DPPH free radical scavenging and betacaroten:linoleate assays. The phenolic compounds fractions were determined using LC-MS system. TPC of extracts ranged between 1502.25 to 1836.69 mg GA/100g E. The results showed that SFE and UAE have highest extraction yield and TPC respectively. The UAE extract has highest antioxidant activity in DPPH free radical scavenging method. Clorogenic acid (13.54%) was the most compound of extract. The results of this study suggest that pimpinella extract due to have phenolic compound have antioxidant and antimicrobial activity and it can be good alternative for synthetic antioxidants.