Effect of biopriming with Trichoderma and Pseudomonas on germination and some biochemical characteristics of deteriorated flax (Linum usitatissimum L.) seeds CV. Norman

Seed deterioration as an irreversible physiological phenomenon, have significant effect on seed quality. Increasing and maintaining of seed quality by using methods such as biopriming is important. For this reason a three-factors experiment was conducted on one linseed cultivar (Norman) based on randomized completely design with four replications, in laboratory of Seed Science and Technology, college of agriculture, Yasouj university in 2015. Factors were includeing storage period (31, 53, 93 and 130 days); storage condition in two combinations of storage temperature (°C) and seed moisture content (%) (moderate condition (15°C/17) and severe condition (35°C/9%); and biopriming with two strains of Thrichoderma harzanium (T36 and T40) and two strains of Pseudomonas fluorescens (CHA0 and P.F(2)) and non-priming (control). Seeds inoculation by bacterial and fungal suspension was done before or after storage. During storage period of 31 to 130 days, germination parameters and peroxidase enzyme activity decreased and electrolyte leakage, soluble sugar content and malondialdehyde content increased. During 31 and 53 days of storage under moderate condition biopriming with T40, P.F2 and CHA0 have increased germination indices compared to non-primed seeds. In 93 and 130 days storage treatments, priming with T40 significantly affected germination compared to other treatments. On the basis of current study using effective biological treatments in improving germination and maintaining Flax seed quality under different storage condition is suggested.