Insilico Systems Biology Explorations of Escherichia Coli's Metabolic Network and Its Application in Mastitic Dairy Cattle

Mastitis is an important disease that could turn up in both milking and dry off periods in dairy cattle. Considering the importance of Escherichia coli in causing microbial mastitis, this study in an Insilico way was undertaken to investigate the amount of biomass, toxin production and to identify genes affecting on Escherichia coli growth in both aerobic and anaerobic conditions in alveoli of mastitis dairy cow. The optimum rate of Escherichia coli growth or biomass production adopting aerobic condition was 2.0155 millimoles per gram dry weight per hour (mmol/gDW/h) but in anaerobic condition this value was 1.0152 mmol/gDW/h. The most toxin production was obtained in aerobic condition (1.6701 mmol/gDW/h). This substantial amount of biomass production could be a great indicative of rapid growth of Escherichia coli and therefore, inflicting the mastitis in dairy cow. Multiple gene deletion indicated that four genes ompF, ompN, PhoE and ompC brought about of stopping biomass production. Also, it was indicated that solA, LpxM ¡ ZnuA and znuB genes are suitable drug targets for reducing or stopping toxin production in Escherichia coli.