Phylogenetic characterization of chicken infectious anemia virus detected from broiler chickens in Chahrmahal VA Bakhtiari province, Iran

Chicken infectious anemia virus (CAV), cause severe anemia, and makes atrophy in lymphatic organs include thymus, bursa of fabricious, spleen and bone marrow. It suppresses immunity system and increase the susceptibility to other infections. Subclinical infection of CAV caused high economic losses in broiler flocks. Isolation of the virus in tissue culture, detection of genome by PCR based method and serological tests are diagnostic methods of chicken infectious anemia virus. The aims of the present study were detection and partial nucleotide sequencing of CAV genome by PCR and direct sequence method, respectively. Seventy seven liver samples from chickens belong to 8 different broilers flocks around the Sharekord city, in Chahrmahal VA Bakhtiari province, were tested. Results showed that 19 samples belong to 4 broiler flocks were positive. Amplicons of 4 PCR positive samples belong to 4 different flocks were sequenced and the results showed that partial gene sequences of chicken infectious anemia viruses had 100% homology. These sequences had more homology with nucleotide sequences of Indian, Malaysian, German and Chinese CIAV strains. With regards to phylogenic similarity, it seems that all of these isolates have a same origin.