Reaction of various explants of a Chrysanthemum morifolium cultivar to plant growth regulators in vitro

Economic importance of Chrysanthemum among the other ornamental species has resulted in performing various investigations in tissue culture optimization of this plant, worldwide. In the current experiment, leaf, petiole, petal and stem TCL explants, from Chrysanthenum morifolium cv. Reagan Elite Salmon were cultured in MS medium supplemented with different combinations and concentrations of NAA, BAP and TDZ. Regeneration percentage,number of regenerated shoots per explants and regeneration type were investigated in each treatment. Results indicated significant superiority of shoot regeneration percentage from petiole explants (80.5 percent), while minimum regeneration was achieved from the petal explants (37.2 percent). Shoot regeneration type from different explants in the treatments containing only BAP, was direct and in the treatments containing TDZ and NAA was indirect. Reactions of the explants to the PGR treatments, considering shoot regeneration percentage and average number of regenerated shoots per explants, were so different. As among the treatments consisting combinations of NAA plus each of the cytokinins, in T12 (4.5 mg/l BAP 1 mg/l NAA), petioles and petals represented maximum shoot regeneration percentage and number, while stem TCLs showed minimum rates of regeneration.