Molecular detection of 16s rRNA, qacA / B, smr in Clinical Isolates of staphylococcus aureus methicillin resistance

Disinfectants and antiseptics agents of the past were used for disinfect equipment and surfaces. In some cases, these substances ineffective by a series of specific efflux proteins that are present in bacteria. QqcA / B and smr such as most important genes those can be expression more efflux protein in strain of methicillin-resistant staphylococcus aureus (MRSA). The aim of this study was to investigate the genes qacA / B and smr is MRSA strains.
89 isolates from various clinical samples were early identified by biochemical test. Initial screening was performed by Cefoxitin disk diffusion (30 µg) and then detection of mecA, smr and qacA/B gens was done by polymerase chain reaction (PCR). Finally, were analyzed results by using chi-square tests and t-test.
Out of 89 staphylococcus aureus isolates, 59 isolates were identified methicillin-resistant by initial phenotype test. Among those, 50 isolates (17/56%) have respectively mecA gene, 14 isolates (73/15%) smr gene and 29 isolates (58/32%) qacA / B gene. Using by statistical tests and p.value test, was obtained the relationship between qacA / B and mecA genes.
According to the results of the studie of prevalence of genes in Zahedan and high frequency of MRSA strains, should pay more attention to the use of disinfectants agents.