Expression Analysis of Rheb Gene in Different Tissues of Jabal Barez Red Goat

According to global, regional and economic importance of Jabal Barez Red goat from the cashmir production aspect and role of Rheb gene on growth, cell cycle and cancer, expression of this gene was studied in Jabal Barez Red goat for the first time. As one member of the Ras super family, Rheb is an upstream regulator of mTOR signaling pathway, which regulates the process of cell-growth, proliferation and differentiation. In order to study expression levels of Rheb in various tissues including brain(medulla), brain(cortex), heart, kidney(cortex), kidney (medulla), testis, lung, liver and spleen,the cDNA of Rheb gene was amplified by Real-Time PCR and analysed by Pfaffl method. SAS software was used to analyze the resulted data of the Pfaffl method. Results showed that the Rheb gene was expressed in the all tested tissues and the highest level of expression was observed in spleen and the lowest level in lung. Hence, it can be suggested that Rheb gene express in all tissues that require the physiological effects of this gene to be considered in different tissues and different animals.