Investigating of Polymorphism in Intron 1 of Prolactin Receptor Gene in Shal Breed and Shal×Romanov Crossbreed using PCR-SSCP Technique

Prolactin is secreted from the anterior pituitary gland and plays an important role in the mammary gland development, differentiation and lactation. The aim of current study was to evaluate prolactin receptor gene polymorphisms in the Shal breed and Shal×Romanov crossbreeds. For this purpose, after blood sampling of animals and extraction of DNA using phenol chloroform method, two fragments of 176 and 215 base pairs from intron 1 of prolactin receptor gene were amplified using PCR reaction. Then PCR products were analysed by acrylamide gel electrophoresis and silver staining. Results showed that AB and AC genotypes were identified in 176 base pairs fragment of prolactin receptor gene (PRLP1) in Shal breed. The frequencies of these genotypes were 0.92 and 0.08, respectively; and the frequencies of A, B and C alleles were 0.5, 0.46 and 0.04, respectively. Also, in the position of PRLR1 of this gene in Shal×Romanov crossbreeds, the frequencies of AB and AC genotypes were 0.94 and 0.06, respectively; and the frequencies of A, B and C alleles were 0.50, 0.46 and 0.04, respectively. A polymorphism study in the 215 bp fragment of the prolactin receptor gene (PRLR2) in the Shal and Shal×Romanov crossbreeds showed only AA genotype. Results of this study showed that PRLP1 position in Shal breed and Shal×Romanov crossbreeds was polymorphic, however the PRLP2 position in the studied samples was monomorphic.