Evaluation of Nutritional efficiency of encapsulated fish oil ca-salts in vitro and in vivo

This study was conducted to production and evaluation of encapsulated fish oil ca-salts using a saturated FA containing material. Nutrient content and FA profiles of produced supplements were determined and ruminal biohydrogenation and oil release were examined in vitro. Additionally, in a complementary in vivo experiment, effects of dietary inclusion of fish oil, ca-salts and encapsulated ca-salts on nutrient digestibility and ruminal paramerters were evaluated using three rumen fistulated Holstein cows. Encapsulation of Ca-Salts increased protection efficiency of PUFA against ruminal biohydrogenation. Encapsulated ca-salts with 10 % of wall material (weight basis) had lower biohydrogenation afer 48-h in vitro incubation (P˂ 0.05). Encapsulation of ca-salts decreased rumen oil release but oil release in other simulated media or total tract oil release were not affected by encapsulation (P˃ 0.05). Protected supplements increased nutrient digestibility compared with fish oil (P˂ 0.05). Results of ruminal parameter analysis can warrant inertness of ca-salts and encapsulated ca-salts in the rumen of experimental animals. Fish oil decreased acetate and rumen N-NH3 concentration as well as protozoa `population, but increased propionic acid concentration (P˂ 0.05). Encapsulation of Ca-Salt did not changed rumen parameters (P˃ 0.05). According to the results, it can conclude that encapsulation of ca-salts increased protection of PUFA against ruminal biohydrogenation without worse effects on rumen parameters. Encapsulation with 10 % of wall material (weight basis) can be concluded as the best treatment according to chemical composition, fatty acid profiles, rumen biohydrogenation and oil release data.