Investigation into micropropagation of Narcissus tazetta L. from leaf explant

Narcissus tazetta is one of the most important ornamental bulbous species endemic to Iran. Micropropagation of Narcissus tazetta is an important method for rapid propagation and disease elimination of these plants. The aim of this research was to determine the optimal conditions for micropropagation of this plant using leaf explant. To conduct the experiment the sterile leaf explants were cultured on MS media containing BA with IBA or NAA at different concentrations. All media were supplemented with 30 gl־¹ Sucrose and gl־¹ Agar. Results showed that the highest number of shoots (3) with elongated stems (24 mm lenght) were obtained in media containing 2 mgl ¹ BA with 1 mgl ¹ IBA and 4 mgl ¹ BA with 0. 25 mgl ¹ NAA. In this media, the regeneration rate of explants was 100%. In the shoot proliferation stage, shoots were cultured on MS media supplemented with different concentrations of BA and IBA or BA and NAA. The best results were obtaind in the media containing 2 mgl ¹ BA and 1 mgl ¹ IBA in which 100% of explants produced shoots. In this medium, the highest number of shoots per explant (mean 3) and the highest shoots (mean 20 mm lenght) were produced.