In vitro optimization of the brown algae (Sargassum glaucescens) extract Antioxidant properties using Response Surface Methodology

Marine algae, Sargassum glaucescens, collecting from Chabahar coastal waters in order to investigate the antioxidant properties of the extract. Extraction was set at three levels of time (1, 3 and 5 hours), temperature (24, 47 and 70 °C) and solvent concentration (60, 80 and 100%). Antioxidant properties were measured by DPPH free radical scavenging method. In this research, prediction of the antioxidant properties of Sargassum algae was done using optimization through response surface methodology. Based on the results, the conditions of optimum extraction treatment were determined by the Method as: the concentration of %99.39, the temperature of 57.66o C and 4.32 hours for the extraction time. In this method, the highest level of antioxidant properties was determined as % 36.9334. The amount of total phenolic content for the Sargassum glaucescens was 3.2±0.56 per mg of galic acid /g of extract. The results showed Sargassum glaucescens algae could be a good source for the extraction of antioxidant compounds.