Effect of CPP-ACPF on Microhardness of Enamel before and after exposure to energy drink (in vitro)

One of the most regular extrinsic factors that decreases microhardness of the enamel is acidic beverages such as energy drinks. These beverages reduce the pH of the oral environment and could solve dental enamel. This in vitro study demonstrates the effect of CPP-ACPF paste on enamel microhardness, demineralized by energy drinks.
In this experimental study, 30 intact enamel pieces of human premolars were selected and primary microhardness was measured and recorded on all samples by Microhardness Tester (Vickers). Then divided into three groups. Control group was immersed in 30 ml Red Bull drink and artificial saliva. This procedure was for 7 days. Group 2 were immersed in Red Bull drink like group1, but samples were coated with 0.3 gr CPP-ACPF for 3 minutes after immersing in energy drink. Then the paste was mixed with artificial saliva for 2 minutes. In group 3, first, CPP-ACPF was applied similar group 2, then after one hour, the samples were immersed in Red Bull drink as mentioned before. At the end of 7 days, the secondary microhardness was measured. The data were analyzed with repeated-measure ANOVA and Post Hoc analysis.
The microhardness was significantly different before and after the interventions in each group (P According to this study, Red Bull energy drink decreases enamel hardness. Using CPP-ACPF paste before or after drinking energy beverages could increase the hardness and improve remineralization.