Efficient production and application of egg yolk antibody powder against Staphylococcus aureus

The aim of present experiment was to investigate the immunochemical characteristics as well as in vitro inhibitory effects of specific and nonspecific egg yolk antibody powder on Staphylococcus aureus growth, as one of the main pathogenic serotypes of Staphylococcus in human and animals. A total of 16 White Leghorn hens (Hy-Line W36) of forty weeks in age were immunized by killed sonicated vaccine after plotting the growth curve and incubating the bacteria until the end of exponential growth phase. This vaccine was prepared eggs were collected daily for 8 weeks, IgY was then isolated and lyophilized by Polson method. The immunochemical properties and growth inhibitory effects of specific and nonspecific sIgY were tested against Staphylococcus aureus. The results showed that the plasma and egg specific activity of IgY against Staphylococcus aureus markedly increased after the first immunization and remained on the highest titer throughout the experimental period (P