Synthetic hydrated seed production in medicinal plant of Chavyl (Ferulago angulata L.) through encapsulation of somatic embryos

Artificial seed production can be considered as an alternative method to overcome the problems of cultivation, propagation and conservation of medicinal plants. For this purpose, artificial seed production using somatic embryos of two genotypes of Chavyl (Ferulago angulata L.) (Chehlcheshme and kohgol) was evaluated in two separate experiments. In the experiment of investigation of the number of somatic embryos ( the first test ), after observing the torpedo embryos , number of globular , heart and torpedo embryos formed on the surface of embryogenic callus were counted that the significant difference was not observed between the two genotypes in the number of somatic embryos . In the artificial seed production experiment (second experiment) produced somatic embryos were sealed using calcium alginate capsules. In this way that somatic embryos were transfered to liquid full Murashige and Skoog growth medium (MS) and mixed with sodium alginate 2 and 3% and were dropped in calcium chloride solution, calcium alginate capsules were formed within 10 and 30 minutes. The results of this experiment showed that for Chehlcheshme variety complex treatment of sodium alginate 2% and calcium chloride 25 mM for 30 minutes and for Koohgol variety complex treatment of sodium alginate 3%, calcium chloride 50 mM for 30 minutes were good to produce artificial seeds with maximum germination.