Effect of dietary conjugated linoleic Acid (CLA) Supplementation with injectable Se and VE supplement on productive performance and blood parameters of holstein dairy cows

Conjugated linoleic acid refers to the positional and geometric isomers of linoleic acid (cis–9, cis–12 18:2). The CLA have conjugated double bonds that are separated by a single bond between 2 carbons. Generally, two isomers are addressed with respect to CLA is cis-9, trans-11 CLA and the trans-10, cis12 CLA., the studies have shown that Trans-10, cis-12 is a specific CLA isomer that decreas milk fat synthesis in lactating cow's supplements may improve energy balance (EB) in lactating cow's by reducing their energy requirements for milk synthesis. One the other hand with improvement in method of of fat from biohydrogenation in the rumen use of aitioxidant,s is nesseray when protected unsatturated fat are fed. Vitamin E (VE) and (Se) are essential micronutrients that share a common biological role as antioxidants. The purpose of this expperiment was to evaluate effect of Supplementary Rumen protected CLA with Supplementary injection of E-Se on reproductive perfomance and blood metabolit's change in Holstein cow's in transition period and early lactaction
forthy multiparous dairy cows were seleced and alloated randomaly into four treatment groups based on their previous milk yield and expected calving date during 21th precaving until 60th post calving .treatment groups consisted of 1 basal diet (Control), 2-basal diet plus injectable E-Se Supplement (E-Se),3- basal diet plus CLA 100 g/day per each cow (CLA) 4- basal diet plus CLA 100 g/day per each cow and Injectable E-Se Supplement (CLASe.The dose of injectable E-Se supplement was 40 ml/cow. The injection has done muscularly in each cow in days, -21, 1, 14, 28 and 42 after calving.. Dry matter intake an milk yield daily, milk composition weekly, 'Weight and BCS Three weekly was measured. Blood samples were collected in -21, , 1, 7, 14, 21 periods of calving.
feeding CLA had significantly affected on increase of milk yield 'milk efficiency and energy balance in dairy cow's (P0.05). althought protein yield, Solid not fat and lactose, significantly increase with CLA supplementation (P0.05). Feeding CLA had not significant affected on blood metabolit's in caving and post calving.interaction effect's of CLA with E-Se had siginificant on glucose concentration (P Results of this experiment showed that use of CLA supplement to dairy cows cause decrease in milk fat and reduce BCS losses and incerase milk yield and improvement in energy balance but had not significant effect on blood parameter. use of injectable E-Se supplement cause decrease in milk fat yield and increased milk SNF yield use of CLA supplement and injectable E-Se supplement non signifiantly improve reproductive performance of dairy cows