ANALYSIS OF P27KIP1 PROMOTER METHYLATION IN PATIENTS WITH ULCERATIVE COLITIS
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background and Aims
The CDK inhibitor (CDKI) protein p27kip1 (p27) negatively regulates cyclin DCDK4 complex in the G1 phase. Alterations in the expression of p27 kip1 cause a degradation of cell growth and promote the development of various diseases. Aberrant methylation patterns have been reported in large number of diseases. The purpose of this study was to investigate the methylation pattern of p27 kip1 gene promoter in patients with ulcerative colitis (UC) and normal subjects.Materials and Methods
Methylation specific PCR (MSP) was performed on 230 normal controls and 125 samples of patients with UC. Statistical analysis was performed using MedCalc software.Results
The p27kip1 promoter was methylated in 16.8% (21/125) of UC and in 4.8% (11/230) of normal samples. The difference in p27 kip1 methylation between the UC and normal groups was statistically significant (PConclusion
Methylation of p27kip1 may contribute to UC development. However, further studies with larger sample sizes are needed to confirm this result. Keywords:
gene silencing , methylation , p27kip1 , p27 , UC
Language:
Persian
Published:
Journal of Medical Science Studies, Volume:29 Issue: 5, 2018
Pages:
372 to 380
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