Evaluation of Radiated Potato Genotypes with Gamma Rays in water Deficit Stress

This research was conducted in order to induction of genetic diversity through radiation with gamma rays in potato cultivars for tolerance to deficit water stress under in vitro condition in Karaj Nuclear Agricultural Research Institute and Laboratory of Zar-Gostar-Arta Technology Company, during 2016 and 2017. From each cultivar of Jeli, Esprit, Banba, Milva and Agria, 3000 plantlets reproduced and were irradiated with 25 Gary of gamma rays. After evaluation, 2517 genotypes were survived. The first experiment was factorial based on completely randomized design with three replications. The first factor was potato genotypes and the second factor consisted of four concentrations of poly-ethylene-glycol including 0, 150, 220 and 275 g.l-1. After evaluation under normal and water deficit stress, 66 genotypes were selected. In second experiment 66 selected genotypes and 5 cultivars as control totally 71 genotypes were conducted based on factorial design based on completely randomized design with three replications. The first factor was 71 potato genotypes and the second factor consisted of four concentrations of poly-ethylene-glycol including normal (0 g.l-1), mild (150 g.l-1), severe (220 g.l-1) and very severe (275 g.l-1) and were investigated in terms of plant height, leaf number per plantlet, root number per plantlet, node number per plantlet, internode length, micro-tuber number and weight per plantlet and micro-tuber average weight. The Esprit-G8 genotype under normal and mild condition and Milva-G3 genotype under severe condition had the highest micro-tuber weight per plantlet. In normal condition, Esprit-G8 genotype and under mild and severe conditions, Milva-G1, Milva-G3, Banba-G5, Banba-G6, Banba-G7, Banba-G9 and Esprit-G8 genotypes had the highest in terms of leaf, node and micro-tuber number per plantlet and were selected as tolerant genotypes to deficit water stress.