Effects of Oral Creatine Supplementation on Serum Levels of BUN, Creatinine and Renal Tissue in Trained Male Rats

The use of creatine (Cr) as a nutritional supplement to aid athletic performance has gained widespread popularity among athletes. However, it has been recently expressed over the potentially harmful effects of short and long term creatine supplementation on health. The aim of present study was to the effects oral creatine supplementation on serum levels of BUN, creatinine and renal tissue in trained male rats.
Material & In this study, Wistar male rats weighing 245±5g were divided into five groups (n=8): control, sham, exercise with low-dose creatine (200 mg/kg/d), and exercise with moderate doses of creatine (300 mg/kg/d), exercise with high doses of creatine (600 mg/kg/d). Blood biochemical studies were performed after ten years of supplementation and exercise. Following serum collection, levels of BUN and creatinine were measured by spectrophotometry method. Statistical analyses were performed using SPSS and mixed model of ANOVA.
Findings: The results indicated that creatine supplementation at the doses given produced 200,300 and 600 mg/kg/d no significant changes in serum level of BUN compared to control group (p>0.05) and at the doses of 200 and 300mg/kg/d no significant changes in serum level creatinine compared to control group (p>0.05). But, the high dose creatine diet exercied group showed significantly higher serum level of creatinine (pDiscussion & The results indicated that high dose