Effects of Apium graveolens extract on serum calcium and oxalate in ethylene glycol- induced kidney injury in male Wistar rats

Celery (Apium graveolens) is a biennial, belonging to family Apiaceae. Various parts of celery are used in hepatic and spleen disorders, brain disorders, body pain, and sleep disturbances. The present study was planned to investigate effect of celery ethanolic extract on ethylene glycol induced kidney injury in male Wistar rats. Urolithiasis was induced in hyperoxaluric rat model by giving 0.75% ethylene glycol for 28 days. Animals were divided into 8 groups (n=6) including normal control, urolithiatic control (0.75% ethylene glycol in their drinking water), normal rats receiving celery extract at doses 50, 100, 200 mg/kg, b urolithiatic rats receiving celery extract at doses 50, 100, 200 mg/kg intragastrically. In the end of experiment (28 days), blood samples were collected by cardiac puncture and serum calcium and oxalate was analyzed. Statistical analysis was performed by one-way analysis of variance followed by Tukey post hoc test (p<0.05). Our results showed that significantly elevation of serum calcium and oxalate in urolithiatic control compared with the normal control rats. However, administration of celery extract produced significantly reduction in serum calcium and oxalate in urolithiatic rats compared with the urolithiatic control. Thus, celery extract could ameliorate urolithiasis induced by ethylene glycol. Therefore, celery extract might have potential clinical implications in preventing the oxidative renal cell injury that can ultimately lead to kidney stone formation.