Study the effects of auxins and cytokinins on growth , pigments and protein contents of Chlorella sorokiniana

Chlorella are used as medicine, aquatic foods and biofilter. For this reason increases of its biomass and some of metabolites have economic value. The aim of this research was study of stimulating effect of some phytohormones on biomass and important metabolites contents of Chlorella sorokiniana. In this study C. sorokiniana was cultured at modified Bold Basal Medium. Glucose monohydrate (5gl-1) and different proportions of plant hormones naphthalene acetic acid (NAA), benzylaminopurin (BAP), indolebutyric acid (IBA) and indoleacetic acid (IAA) were added to medium. Dry weight, pigments and protein contents were measured. At 75% of NAA+BAP treatments, these growth parameters increased. At treatment with NAA (10mgl-1) +BAP (1mgl-1), these parameters significantly enhanced compared to control. At treatment with IAA (1mgl-1) +BAP (2mgL-1), dry weight and content of chlorophyll a, significantly increased compared to control. At treatment with IBA+BAP, dry weight and metabolites had not significant difference or reduced compared to control. The highest proportion of dry weight, cell numbers and pigments and proteins contents were observed at NAA+BAP treatment. Results showed BAP, NAA and IAA had the greatest additive effects on growth traits respectively, but IBA had little effect on these traits