Determination of amounts, antioxidant properties and identification of main phenolic compound in Enarijeh(Froriepiasubpinnata)extract by RP-HPLC method

Enarijeh(Froriepiasubpinnata) is an endemic plant with widespread distribution in woody region in the North of Iran.This study was conducted for the first time to evaluate antiradical antioxidant properties of Enarijehextract using different tests (Folin-Ciocalteu, total antioxidant capacity, DPPH and ABTS free radicals scavenging as well as reducing power), identification, and measuringpredominant phenolic acid in the extractusing high- performance liquid chromatography.All the evaluations exhibited appreciable antioxidant potential for the extract(p< 0.05). It showed various degrees of efficiency in each assay in a dose-dependent manner.Total antioxidant activities of Enarijeh extract in concentrations less than 0.03% were more than BHT asa synthetic antioxidant. So that in theconcentration of 50 μg/ml the antioxidant ability of the extract BHT was 0.47 and 0.22 μg/ml, respectively. It should be pointed out that the occurrence of chlorogenic acid (5-O-caffeoylquinic acid, 5CQA) as predominant phenolic acid in Enarijeh was detected for the first time. On the basis of obtained results, Enarijehmay be introduced as a rich novel source of natural antioxidants for food drug industry.