Investigation of Oleuropein Lipid Nanostructures Using Dynamic Light ScatteringTest (DLS) and Differential Scanning Calorimetry (DSC)

Polyphenol compounds are sensitive to environmental conditions while show beneficial feature asnutraceutical, hence designing targeted delivery systems with controlled release of this compound through microencapsulation in various matrices, is one of the solutions to minimize these disadvantages. In this study, glycerol mono stearate, soybean oil and linoleic acid nanoparticles aswalls, lecithin as emulsifiers and olive leaf extract powder (varieties of Spanish and Khorramabad) was chosen as the core material to prepare nanoparticle. The nanoparticles were prepared using modified method of emulsification diffusion and the particle size, zeta potential and particle dispersion index of them were evaluated.The results showed that the optimal condition for extractionobtained using mixture of ethanol and water (70:30), which yield the highest amount of polyphenols (oleuropein). The results of light scattering showed that the formula contains 0.05 gram extractedpowder has the lowest average particle size and particle size distribution. The results of Zeta-sizer showed that the zeta potential of nanoparticles inoptimal formula (0.05 gramspowder) is -43.3 mV, which represents the physical stability of formula. In evaluatingthermal properties of the different treatments it was showed that oleuropein in combination with lipid materials in the nanocomposite has changed the nanocomposite thermal properties.