Antileishmanial Effect of Plant Extract alpha-pinene (Pistacia atlantica) In vitro and In vivo

Leishmaniasis is a zoonotic disease which presents with a wide range of clinical features, including cutaneous and visceral forms in Iran. Leishmania (L) major is one of the agents responsible for cutaneous leishmaniasis transmitted by the bite of sand fly. In this study we assessed the antileishmania effect of pistacia Atlantica (alpha-pinene) in culture and also its therapeutic effects on Balb/c mice infected with L. Major.
Material and Certain amount of promastigots was challenged with increasing concentrations of pistacia atlantica extract. MTT test was used to assess promastigote survival after 24, 48 and 72 hours. For in vivo assessment, in the stationary phase, 0.1 ml solution containing 2×106 promastigotes were injected subcutaneously into the base of the tails of the mice. Four weeks after injection, cutaneous lesions appeared and different doses of the extract were applied daily in the form of an ointment for 3 weeks. Diameters of the lesions were measured at the end of each week and therapeutic effect of the extract on the lesions was assessed. The results of MTT test revealed remarkable effect of the treatment on the growth of promastigots. IC50 values for glucantime and alpha pinene were found to be 10 µg/ml and 1.46 µg/ml respectively. 30 % ointment of the extract decreased the lesion diameter significantly while 15% ointment and treatment for control group were ineffective. The results of the present study showed antileishmanial effect of alpha-pinene on Leishmania major promastigots, in vitro. Moreover, topical ointment of the extract can reduce size of the lesions caused by the parasite, in vivo.