Evaluation of effect Schiff base complex Cu2+ N,Nˊ-dipyridoxyl(1,2 diaminobenzene) on angiogenesis process on chorioallantoic membrane and genes expression of VEGF and VEGF-R

Angiogenesis plays an important role in tumor growth and metastasis of cancer; therefore, compounds that inhibit the angiogenesis process can prevent tumor growth. Recently, Schiff base complexes have become important due to properties such as anticancer, anti-oxidant, and the like. In this study, the angiogenesis effect of the copper Schiff base complex on the chorioallantoic membrane was investigated. to do research from 40 egg spatula were used. After 48 hours of incubation, a window was cut on eggs and were treated by Schiff base complex on the eighth day. On the twelfth day, photographs were taken from the samples. The number and length of blood vessels, and then the weight and length of the embryos were measured. Technique of Real Time PCR were used to study the change genes expression of VEGF and VEGF-R on MCF7 cells. The results showed that the Schiff base complex was able to significantly reduce the number and length of blood vessels. Also, by increasing the complex concentration, the weight and length of the embryos decreased. Investigating the genes expression (VEGF and VEGF-R (showed that the Schiff base complex cu (II) were able to inhibit genes expression. The results indicate that this combination can reduce and inhibit the process of angiogenesis. Therefore, it can be introduced as a candidate in treatment to pathological diseases such as cancer.