Investigating the survivine gene expression in DU145 prostate cancer cell line treated by Kombucha extract

Aim and Prostate cancer is the second common malignancy in men. In this study, the effect of Kombucha extract on DU145 prostate cancer cell line viability and expression of Survivine gene expression in cancer cells have been investigated
Material and Cell line was affected by extract of Kombucha and the amount of cell death through MTT and expression of Survivine gene expression by Real Time PCR were evaluated. The results showed that the cells treated with this extract significant had reduction in the number of cells after 48 hours and also had a decreasing effect on the expression of Survivine gene. These effects were dependent on the time and concentration of the treatment. The highest expression loss was observed at 75% concentration of extract in 72 hours after treatment. Kombucha extract, by affecting the main genes in the pathway of apoptosis, reduces the amount of cell division, and this decrease leads to inhibit development of cancerous tissue