Evaluation of antioxidant and antibacterial activities of hemolymph proteins from oriental river prawn (Macrobrachium nipponense )

Recently the use of natural bioactive compounds have been increased due to the importance of them in the prevention of cardiovascular diseases as well as cancers. This study, was carried out on 220 prawn samples which collected from the Caspian Sea in spring 2015. The hemolymph of both genders of prawn separately collected from the abdomen using insulin syringe. After extraction of proteins, anti-bacterial activity of them were evaluated by the Agar disc Diffusion method against the five different bacteria. In addition antioxidant properties of hemolymph were measured with some antioxidant indexes such as radicals scavenging DPPH, hydroxyl, hydrogen peroxide and reducing power.
Highest and lowest inhibition zone diameters 18.27 ± 0.2 and 10 ±0.14 mm against Vibrio cholera and Escherichia coli, respectively. The prawn hemolymph proteins showed significant bactericidal effect against the bacteria (p<0.05). The highest inhibitory activities of DPPH radical, hydroxyl radical and reducing power were recorded 88.2% 57.6% and 29.2% respectively at concentration of 100 µg/ml in male hemolymph. There was significant difference in the inhibitory activities in DPPH scavenge ring and reducing power among genders (p<0.05). The results showed that the prawn hemolymph proteins have bactericidal potential as well as anti-radical scavenging .So it can be used for medical purposes, food supplements after the clinical experiment.