In vitro study on cytotoxic effect of methanolic extract from carpet anemone tentacles against brain and colon cell lines

Carpet anemone, Stichodactyla haddoni is an ocean resident sedentary animal. The venom of sea anemones could be a potential source of bioactive pharmaceutical compounds. The main goal of this research was to study the killing effects of the methanol extract tentacles from the Persian Gulf sea anemone, S. haddoni against Brain and colon cell lines in vitro using tetrazolium salt. The specimens were collected from coastal waters of Lark, island Persian Gulf (south of Iran). From tentacles methanol extract was performed. These cells were exposing in presence of different Serial dilution 100 to 0.78 μg of venom extract in during 24 hour. In this study statistical analysis showed that activity was similar to in almost all doses (Pvalue <0.001).The extraction induced more lethality on brain cell line than the other cancer cell lines. IC50 of crude venom against brain and colon cell lines was observed at 6.58, 31.54µg respectively during of 24 hour. Therefore, Stichodactyla crude can be considered as a strong agent of growth inhibitor against cancer cell lines especially brain cancer.