The study of sperm morphology and some chemical variables in seminal plasma of Barbubs barbulus Heckel, 1847

This study, with the aim of considering the sperm morphology of male producer Barbubs barbulus, was performed in Aquatic Culture Institute in south of the country (Ahvaz, Sheiban), and 13 fish were studied. After anaesthetization of producers with MS222 and taking sperms through frictional way by a microsampler from 1ml fresh samples inside the 1.5 ml micro tubes , these samples were centrifuged. Seminal plasma, placed over the vial (super net), was measured through separation of top liquid plasma volume, and PH was measured by a PH-meter . After dilution with water, using a chronometer ,the duration of sperm movement was measured by a microscope equipped with a monitor. For study, sperm density was computed after diluting it in a 1:2000 ratio with distilled water, using hemositometeric standard method. To study the morphology of sperm , a microscope equipped with a monitor was used. A photometric method was used for measuring cholesterol , protein, calcium glucose and phosphor. Ion selective method was used to measure sodium and potassium concentration of seminal plasma. In live collected samples, the average weight was 1433±85.73 gr and average length was 51.85±1.55 cm. In this research the average duration of sperm movement is 332.31±126,37 s, sperm density average was 31.67±12.78, PH average of seminal plasma was 8.05±0/193, and average percentage of spermatocrit was 66%, the average of glucose, cholesterol, and protein of total seminal plasma were 15.39±5.86 , 50±21.2 and 1.66±0/86 mg/dl respectively, the average of Na, Mg, Ca , and P in seminal plasma were 434.15±72.8, 112.39±23.8, 3±0/7, 0/75±0/3 and 16.25±2.4 mlmol/l respectively, as well as the average length of sperm head was 0/66± 0/13mic, the average length of cilium(cirrus) was 2.04±0/29 mic and the total average length of sperm was 2.71±0/32 mic. Duration of movement period had a meaningful relationship with sperm density, seminal plasma PH, glucose and the percentage of spermatocrit (P