Studying and identification of agaric fungi in oak forests of Kermanshah province

Agaric fungi are a large group of Basidiomycetes that known as mushrooms in the broad sense. In order to identify and characterize the agaric fungi in oak forests of Kermanshah province, specimens were collected in 2014-2015. Microscopic and macroscopic characteristics including size and color of cap and stem, size and shape of basidia, the number of strigma, size, shape and color of spores and cystidia type were checked. Both morphological and ITS sequencing were used for identification. DNA extraction was carried out using a Genomic DNA Purification kit. The ITS1, 5.8S, and ITS2 (ITS) Regions were amplified using ITS1 and ITS4 primers. Fragments about 600 bp were amplified and after sequencing deposited in GenBank. Based on both morphological and molecular characteristics Agrocybe praecox (KT833856), Cyclocybe cylindracea (KT833863), Coprinopsis atramentaria (KT833863), Entoloma serrulatum (KT833862), Hebeloma alpinum (KT833861), Hypholoma fasciculare (KT833860), Lactarius glaucescens (KT833859), Lepiota cristata (KT833859), Pholiota gummosa (KT833858), Psilocybe atrobrunnea (KT833864), Psilocybe cyanescens (KT833857), Stropharia aeruginosa (KT833865). Boletus erythropus and Irpex lacteus were identified in Kermanshah province. Hebeloma alpinum, Entoloma serrulatum and Cyclocybe cylindracea are new for Iran mycoflora. All representatives were deposited in fungal collection of Plant Protection Department, Razi University, Kermanshah, Iran.