Evaluation and comparison of microleakage of CEM cement apical plug in three different media

Proper seal is needed for successful endodontic treatments such as apical plug treatment. Some of studies have been shown that tissue fluid can affect the sealing ability adversely. The purpose of this study was to evaluate the sealing ability of CEM cement apical plug in dry, blood contaminated and saliva contaminated canals. 70 single root and single canal extracted human teeth were decornated from CEJ. preparation of apical area were done up to #40 K-file using step back method and coronal area was enlarged up to #80 k-File. After open apex condition reconstruction and creation of dry, blood contaminated and saliva contaminated canal condition, CEM cement apical plug was inserted into the canal. After full setting of CEM cement, the remnant of canal space was obturated using gutta percha and AH26 sealer. Microleaksge value was evaluated with fluid filtration method and data were analyzed using and kruskalwalis and Mann-whitney tests. Based on our data, the microleakage value was 26.96, 44.36 and 27.00 in dry, blood contaminated and saliva contaminated canal, respectively. The micro leakage value in blood contaminated condition was significant lesser than dry and saliva contaminated condition ( P-value= 0.000) It seems that however, sealing ability of CEM cement reduce in blood contaminated condition; but dose not affected by saliva contamination. Of course, more invitro and animal study are recommended to confirm these results.