Effect of multi enzyme and bacterial inoculant on chemical composition and in vitro fermentation parameters of ensiled wilted barely

This experiment was aimed to study the effect of multi enzyme and bacterial inoculant on chemical composition and fermentation parameters of barley silage. Robovio multi enzyme was added at three levels, 0, 500 and 1000 mg/kg of barley dry matter (DM) and Prosage bacterial inoculant (a mixture of Lactobacillus buchneri,Lactobacillus plantarum, Enterococcus faecium and Propionibacterium acidophilus) was added at three levels, 0, 5 and 10 mg/kg of silage DM. Chemical composition, in vitro gas production, protozoa population and protein fractions of experimental silages were measured. Results showed that multi enzyme addition decreased NDF (P<0.05), pH, ash, B2 and C fractions (P<0.01) and increased A and B3 fractions (P<0.01) compared to the control. Barley silage containing 5 mg bacterial inoculant had greater DM, pH and B2 fraction (P<0.01) and lower C fraction compared to the control and silage containing 10 mg of bacterial inoculant. Protozoa population, N-ammonia concentration, estimated metabolizable energy, short chain fatty acids and in vitro organic matter digestibility were not affected (P>0.05).  Based on these results use of bacterial and multi enzyme additives for the preparation of wilted barley silage is not recommended due to their costs.