The Cytotoxic Effects of Ethanolic Extract of Leaf and Flower Buds of Crataegus melanocarpa on Human Breast (MCF-7) and Cervical (HeLa) Cancer Cell Line

In the last few decades, research has been focused on the use of natural products for cancer therapy, such as crude plant extracts or some of different plant secondary metabolits. The objective of this study was to examine the invitro cytotoxic activities of crude ethanolic extract of leaf and flower buds of Crataegus melanocarpa, on human MCF-7 and HeLa cell lines, with different concentrations, by Dye exclusion and Micro culture tetrazolium test (MTT) assay. The optical density (OD) colored solution was quantified at 570 nm wavelengths by an ELISA Reader, after 24, 48 and 72 h incubation. The results showed that ethanolic extracts, after 48h incubation, at in 5 and 10 mg/ml suppressed the proliferation of cancerous MCF-7 cells by significant diffrence with control group (p