The antibactrial and antioxidant activity of several types of beepropolis collected from different geographic regions of Golestan province

Propolis is one of the byproducts of bees. Biological activities of propolis is related to geographical and botanical origion. The aim of this study was to evaluate the antibacterial and antioxidant activity the three types of propolis with different geographical origion from Golestan province in northern Iran.The evaluation of antibacterial activity by using agar well diffusion method was performed. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of propolis samples were determined by using broth macrodilution tube method. Also the total phenolic content (TPC), total flavonoid content (TPC) and free radical scavenging activity of propolis samples were determined. Grampositive bacteria compared to gram-negative bacteria showed more sensivity to different concentrations of propolis samples. As MIC of propolis collected from the geographic region of the Shast Kola for B. cereus and S. aureus was, 0/048 and 0/19 mg/ml respectively and for S. dysentery and E.coli 3.12 and 3.12 mg/ml respectively. Propolis sample of JahanNama area with mean of 142.67 mgGAE/g sample and 35.24 mgQE/g sample showed highest TPC and TFC. The highest DPPH radical scavenging activity was related to Shast Kola propolis sample. The antibacterial activity of propolis samples had direct correlation with phenolic
and flavonoid content. The total phenolic and flavonoid content had negative correlation with free radical scavenging DPPH, explain that the antioxidant activity of propolis also is related to other components. So the identification of other compounds of propolis is recommended.