In vitro evaluation of cholesterol lowering effects of isolated Lactobacillus probiotics from cattle and buffalo feces

In this study, the effect of different species of isolated Lactobacillus from cattle and buffalo feces from some Urmia suburban farm were investigated on cholesterol reduction efficacy in
the test tube. Bacteriological studies on cow fecal sample showed that 5 different species of Lactobacillus were able to grow on MRS agar medium. The most frequently isolated species was Lactobacillus rhamnosus (37/5%) and Lactobacillus fermentum and lactobacillus delbrueckii were the less abundant organisms (5%). Bacteriological examination on buffalo fecal sample revealed, ten different species of Lactobacillus and one species of Bifidobacterium were isolated. The higher and lower frequency microrganisms were Lactobacillus acidophilus (25%) and Lactobacillus bulgaricus (2/28%) respectivelly. In this study, the ability of bacteria isolated from fecal samples to reduce cholesterol level in MRS broth medium was considered. It’s should be concluded that non of isolated Lactobacilli from cow fecal samples could modify cholesterol level in test tube, while some of bacteria isolated from buffalo feces could reduce cholesterol level in medium after 24 hours. Depended on bacteria species, this reduction is variable, and the most reduction related to Lactobacillus paracasei (50%), and the less reduction related to Lactobacillus casei (12%).