Lipid peroxidation and sperm parameters in Balb/c male rats treated with different concentrations of Diazinon

Regarding the possible role of Diazinon (DZN) to induce free radicals production, it is assumed that it can lead to increased level of lipid peroxidation and then poor sperm quality. In this research, we aimed to investigate the toxic effects of DZN on sperm parameters quality as well as lipid peroxidation level in sera and seminal plasma of male rats. In this experimental research, 24 male rats (Balb/c) were randomly divided into four groups including: controls (n=6), treatment group A (n=6), treatment group B (n=6) and treatment group C (n=6). Commercial DZN was injected orally in a single dose (A=5mg/kg, B=10mg/kg and C=25mg/kg per day). After 5 weeks, animals were sacrificed for Malondialdehyde (MDA) and parameters quality examinations using Thiobarbituric acid reaction (TBAR) and microscopic techniques, respectively.  There was a significant reduction in sperm parameters quality in treatment groups, especially in group C and group B, when compared with controls (p<0.001). There was a significant difference for serum and seminal plasma MDA mean levels between study groups of A, B, C and control groups (p<0.001).  DZN leads to poor sperm quality via increasing of serum and seminal lipid peroxidation levels in a dose dependent manner.