Investigating callus induction and regeneration of Zelkova carpinifolia forest species In vitro

Zelkova carpinifolia is one of the forest trees which is threatened by Dutch elm disease caused by Ophiostoma novo-ulmi. Forest reservoirs of this species are going to extinct, rapidly. This study was carried out in order to optimize callus induction and regeneration in Z. carpinifolia forest species. The research was carried out in completely randomized design with leaf and stem explants. The results of micro-propagation on Z. carpinifolia revealed that percentage of callus induction in leaf (82.2%)>Stem explants (72.9%). The optimum medium for callus induction (94.3) was observed on Murashige and Skoog (MS) supplemented with BAP (2 mg/L) and NAA (1 mg/L). Also, the maximum shoot regeneration response (7.97%) from callus was observed on MS medium supplemented with GA3 (0.05 mg/L). MS medium supplemented with IBA (2 mg/L) and NAA (2 mg/L) provided 70% rooting response which was then selected as optimum rhizo-genesis media. Keywords: Zelkova carpinifolia, Zelkova tree, Tissue culture, Calligenesis, Regeneration