Effect of media composition and plant growth regulators on in vitro regeneration of Rosa canina and Rosa beggeriana

Two species of Rosa, Rosa canina and Rosa beggeriana, are widely propagated due to their rapid growth and good fragrance. Considering propagation problems of rose flower through traditional ways, in vitro micropropagation methods are recommended. One of the problems of in vitro cultures of Rosa species is yellowing of leaves. Therefore, in the present study, two experiments were designed in order to increase greenery of leaves in in vitro cultures of R. canina and R. beggeriana. In the first experiment, the effects of modified MS basal medium (containing 8.34 mg/L FeSO4 and 30 mg/L AgNO3) and two types of plant growth regulators, BAP and TDZ, were studied. According to the results, the highest greenery percentage of leaves was observed in the medium containing 1 mg/L of BA and 8.34 mg/L FeSO4 (42.6%). The second experiment was designed to study the effects of two types of iron (FeSO4 and Fe-EDDHA) in MS medium containing, 1 mg/L BA and 0.5 mg/L NAA. Results showed that combinational use of FeSO4 and Fe-EDDHA resulted in the highest greenery of leaves (67.15%), branch length (3.92cm) and dry weight (0.03g). Rooting of regenerated plantlets in solid 1/2MS medium containing 0.25 mg/L BA, 1 mg/L IBA, 0.1 mg/L NAA and 40 g/L sucrose, showed the highest number of roots per plantlet (9.1) and root length (4.03 cm).