Micropropagation Of Medicinal Herb- Centella asiatica (L.)

Centella asiatica (L.) is a popular and highly usable herb in the Middle East, Europe and the United States. It has anti-bacterial and anti-fungal properties, and is also used to treat asthma and bronchitis. In recent years, due to overexploitation, and ineffectual efforts to revitalize this valuable herbal source, the abundance of C. asiatica (L.) in nature has suffered significantly, increasing the risk of its extinction. Therefore, interest in micropropagation of this valuable herb through in vitro plant tissue culture techniques has grown considerably. In this study also, for the first time, the micropropagation of this valuable medicinal plant was carried out through plant tissue culture by nodal explants. In order to proliferation, nodal explants collected from herbal material in Gilan province, after sterilization were cultured in MS medium supplemented with BA 1 mgL-1and IBA 0.1 mgL-1. After 1 month, For studying the in vitro shoot proliferation responses, free-contaminated shoot explants were cultured in 9 treatments of modified MS medium, and MS medium supplemented with BA 0.5 mgL-1, 2ip 0.5 mgL-1 and NAA 0.1 mgL-1 was selected as the best treatment. In the comparison of the effect of 11 different hormonal treatments in MS medium on rooting percentage and acclimatization, the MS medium supplemented with IBA 0.5 mgL-1 was chosen as the best treatment.