Phylogenetic and Haplogroup analysis of native goats of Iran based on nucleotide sequence of HVR1 region of mitochondrial genome

Article Type:
Research/Original Article (دارای رتبه معتبر)
Iranian indigenous goats are considered as one of the most valuable genetic reserves and it is important to keep their genetic diversity. The mitochondrial genome in an ecotype and comparing it with other ecotypes can provide an appropriate mesures of the diversity of the population. The purpose of this study was to investigate the genetic structure and phylogenetic relationships analysis of mitochondria HVR1 in four populations of indigenous goats in Iran including of Sistani, Pakestani, adani and Black Lori goats (Each of them has 4 heads) and compare to the other species of goats in the world. Total DNA extraction was performed using phenol-chloroform method and proliferation reaction was performed using a pair of special primers. Proliferation products were sequenced after purification by Sanger method. All of these sequences with17 sequences of the mitochondrial genome of other non Iranian goat ecotypes obtained from the National Center of Biotechnology Information (NCBI) were used for genetic analysis and phylogenetic tree mapping. Studying of HVR1 region of the mitochondrial genome in different goat populations, 123 polymorphic sites and 16 haplotypes were identified. The analysis of molecular variance showed that 15% of variation belongs to between populations and 85% within populations. All Iranian goat populations used in the present study were grouped in the Haplogroup group A, which were commonly found in world ecotypes. The values ​​of the D and Fs statistics of the Tajima test were negative (-2.14 and -9.55 respectively) in the recent study, which may be due to the small size of the sample size. The results of this study showed that HVR1 region of the mitochondrial genome is a suitable tool for genetic studies and Haplogrouping of Iran and Non Iranian goat populations.
Iranian Journal of Animal Science, Volume:50 Issue: 1, 2019
35 to 46  
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