Evaluation of Antioxidant activity, Total phenolic and Flavonoid content of the extract of Adonis aestivalis L. collected from Lorestan Province

Adonis aestivalis L. belongs to the Ranunculoidae sub family of Ranunculaceae family. Up to now many reports has proven the presence of tetraterpenoids, phenolic compounds and cardiac glycoside in the genus Adonis. The most important mentioned effect of A. aestivalis is it's uses in the treatment of cardiac arrhythmia or sexual problems. The aim of this study was to evaluate the antioxidant activity, total phenolic and flavonoid content of the methanolic (%80) and ethyl acetate extract of A. aestivalis and compare them with each other. The total phenolic content (TPC) of the extracts of A. aestivalis was determined using the Folin ciocalteau assay at the presence of galic acid as standard and also the total flavonoid content (TFC) was determined using spectrophotometer methods at the presence of catechin as standard. Antioxidant activity of the extracts of A. aestivalis was determined upon Ferric-Reducing-Antioxidant power (FRAP) assay. The result showed that the total phenolic content of the methanolic extract was determined to be 607.26±2.35 mg GAE/g sample and total flavonoid content 97.81±0.007 mg catechin equivalent/g sample more than ethyl acetate extract (378.37±8.64 mg GAE/g sample, 89.73±0.003 mg catechin equivalent/g sample, respectively). Also the FRAP properties of the methanolic extract of A. aestivalis was determined to be 3.985±0.001 mg GAE/g sample more than ethyl acetate extract (3.494±0.0004) mg GAE/g sample. The results showed that the methanolic extract of A. aestivalis has high antioxidant activity, total phenolic and flavonoid content. Therefore further comprehensive investigation is required to determine the antioxidant properties of A. aestivalis.