Antibacterial Activity of Methanolic Extract of Some Native Medicinal Herbs of North Khorasan Province on Staphylococcus aureus
Due to the increased antibiotic resistance of bacteria due to the uncontrolled use of antibiotics and the self-permeability of antibiotics, finding effective drugs with less complications is very important. Since traditional plants have good antibacterial properties, we decided to investigate the effect of methanolic extract of some samples from herbal plants of North Khorasan Province on Staphylococcus aureus.
Plants were collected from different parts of North Khorasan and dried in shade. After being powdered in a solvent of methanol soaked for 48 hours, the solvent was evaporated by rotary device. After drying, the extracts were dissolved in DMSO and added to the blanc discs and prepared wells. Then, the discs containing the extract were placed on the medium of the Mueller Hinton agar culture medium, cultured with Staphylococcus aureus, from DMSO as negative control and gentamicin as positive control and incubated for 24 hours and finally, the diameter of the bacterial inhibition zone Measured in millimeters.
The studied plants showed antibacterial properties against gram positive bacteria of Staphylococcus aureus, among which Scutellaria pinnatifida and endocarp of walnut had the most antibacterial activity.
Comparison of the results showed that the diameter of the no-growth zone of Scutellaria and interstitial wood of walnut was the highest in comparison with others, which can be used for the antibacterial properties of these two herbs as an alternative to antibiotics and also natural food preservatives Made.
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