Effect of Bacillus coagulants and Lactobacillus casei Probiotics on Liver Damage Induced by Carbon Tetrachloride and on Liver Enzymes Activity in Male Rats

Chemical pesticides cause liver dysfunction. The study was aimed at investigating the effects of Bacillus coagulants and Lactobacillus casei probiotics on liver damage induced by carbon tetrachloride(CCl4) in male rats. In this experimental study, 40 adult male Wistar rats were divided into 5 groups: control group (normal saline, 0.5 ml/day), sham group (Olive oil, 0.5 ml/day), placebo group (CCl4 in the ratio of 1:1 with Olive oil, 2ml/kg body weight, single dose), and treatment groups (CCl4 and Lactobacillus casei or Bacillus coagulans probiotics(. Liver damage was induced by intraperitoneal injection of CCl4 and probiotic treatment with a dose of 109 CFU for 35 days as gavage. One day after the last gavage, the levels of liver enzymes were measured and the histology of the liver tissue was assessed by hematoxylin-eosin staining. The obtained data was analyzed using one way ANOVA, Tukey test and p4 was significantly higher than that of the control and the sham groups (p<0.05). However, they showed a significant reduction in the treatment groups compared to the group receiving CCl4 (p<0.001). Liver tissue damage was also observed in the group receiving CCl4 compared to the control and the sham groups (p<0.001). The recovery in the liver tissue was observed in the group treated with probiotics compared to the group receiving CCl4 (p<0.05). Bacillus coagulans and Lactobacillus casei probiotics might reduce the destructive effects of CCl4 on the liver tissue through stimulating the secretion of antioxidant and anti-inflammatory compounds.