The effects of endurance exercise training on gene expression of GAP-43 and CAP-1 in cerebellar tissue of male wistar rats

Growth Associated Protein 43 (GAP-43) and Adenylyl cyclase-associated protein 1 (CAP-1) proteins are related to nerve cells restorations. But the role of physical exercise on these two proteins in cerebellum has not been clarified yet. The purpose of present study was investigating the influence of six weeks endurance exercise training on gene expression of GAP-43 and CAP-1 in cerebellar tissue of male wistar rats.

20 healthy male wistar rats were selected and divided to two groups of experimental (n=10) and control (n=10). Experimental group performed aerobic exercise, five days in a week for six weekswith the speed of 11-18 m/min for 10-30minutes. Control group did not have any exercise training program. Real time-PCR and2-ΔΔCT were used to biochemistry analysis. Statistical test of kolmogorov-smirnov was used for  evaluating the data normality, and cosidering the violation of normal distribution, mann whitney u test was used for comparing the groups.

Gene expression of GAP-43 (P=0.002) and CAP-1 (P=0.002) proteins increased significantly in the experimental compared to control group.

Given the important role of GAP-43 and CAP-1 proteins in improving the nervous syetem, it seems that endurance exercise training can play a key role as a non-invasive method in increasing the gene expression of the proteins in cerebellum and so recovering and regeneration of cells and improving cerebelluar function.