Investigation of Nanobacteria contamination in cell substrate used in biological productions in Razi Institute by molecular method

Production of viral vaccines and also drugs products which are derive from cells produced in various biological materials, like cell substrates eg: fertile eggs, primary cells, cell lines, raw materials and additives used in culture media which have animal origin and seed virus which is used for culture have a complicated process. The above processes are naturally vulnerable for contamination to adventitious agents. The Nanobacteria like other adventitious agents can cause contamination through primary materials with animal origin for such a product. The aim of this study was to design a program for PCR which is specific for detection of probable presence of Nanobacteria in cells, especially in cell lines and diploids which is used in vaccine production in Razi institute. For this purpose the cells which are used in following departments, research, production and Quality control for biological products were collected and DNA was extracted for PCR expression on 16S rRNA area from Nanobacteria. Bartonella henselae was used as a positive control. As it was expected the results of PCR products after electrophoresis were positive for above organism and at the area of 558 bp replicated which it confirms the correctness of our experiment. The cells from all departments were negative for Nanobacteria, however for not having enough information for spread and risk evaluation of Nanobacteria in cattle population there are a great need for further investigation.