Study of selenium nanoparticles and sodium selenite supplementation effects on expression of adiponectin gene in pregnant ewes

n recent years, special attention has been paid to insulin resistance mediators, such as adiponectin, that is produced and expressed during conception in placenta tissue, thereby inducing its effects on the growing embryo and the health of the mother. In this study, for the first time, Twenty four-month pregnant ewes within the same age were selected randomly. During the 21 days leading up to birth, oral administration of selenium nanoparticles (Se NPs) with dosages of 0.05 and 0.10 mg/kg B.W. and sodium selenite with dosage of 0.1 mg/kg B.W. was carried out. At the same time the control group was fed distilled water in equal volume. With sampling of the placenta during childbirth, transcription amount of adiponectin gene was determined by RT PCR Real Time based on a comparison assay of 2-ΔΔCt. The results showed that the addition of selenium supplements to the diet of ewes of pregnant caused a significant decrease in the amount of adiponectin gene transcription, and the selenium nanoparticle suppression was significantly superior to sodium Selenite.