The Effect of the Mesenchymal Stem Cell Conditioned Media on Mouse Fibroblast Collagen Gene Expression

Collagen synthesis can help to heal wounds, especially Large wounds. Any treatments that can stimulate collagen synthesis in fibroblasts can accelerate wound repair, so it has clinical value. Stem cells secrete some macromolecules and signals that can have different effects in other cells.

Materials and Methods

In this study, human mesenchymal stem cells were cultured and their medium supernatant was harvested as a medium containing the signal. After determining concentration, they were exposed to mouse fibroblasts. The cell viability was examined and then in the molecular section, by designing specific primers, three The collagen gene was evaluated by Real-Time PCR.


The results indicated a growth-promoting effect using the amount of 20 μg /ml of stem cells conditioned media in the culture medium of fibroblast cells. It was also found in the molecular section, that this concentration could increase the expression of all three selected collagen genes.Discussion and


The results of this study fully confirmed the initial goals, indicating the high power of mesenchymal stem cells in the induction of collagen synthesis, and thus could be a very good candidate for clinical studies.

Article Type:
Research/Original Article
Journal of Sabzevar University of Medical Sciences, Volume:26 Issue:5, 2019
651 - 656  
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