Study of antibacterial effect of essential oil and ethanol extract of the Pimpinella anisum and Artemisia dracunculus on three plant pathogenic bacteria (Erwinia amylovora, Agrobacterium tumefaciens and Pseudomonas syringae pv. syringae)

Essential oils and extracts are suitable compounds as alternative chemicals. Many plants have compounds with control properties that recently the use of herbal products as alternative to chemical pesticides for control of plant-damaging agents is more important and the essential oils and herbal extract are of particular interest. The present study was carried out to investigate the antibacterial effects of essential oil and ethanolic extract of Pimpinella anisum and Artemisia dracunculus leaves on Erwinia amylovora, Agrobacterium tumefaciens and Pseudomonas syringae pv. syringae. This study was done based on a completely randomized design with temperature conditions of 28 ± 1 °C and relative humidity of 65 ± 5%, in dark conditions with concentrations (10, 20, 30, 40 and 50 μl) plus 3 replicates for each concentration of essential oil and extract with control At one and two weekly visits. The results showed that antibacterial effect of essential oil and ethanol extract of Pimpinella anisum and Artemisia dracunculus on bacteria Erwinia amylovora, Agrobacterium tumefaciens and Pseudomonas syringae pv syringae were significant compared to the control. Also, whatever the concentrations of essential oils and extracts used in this study increased, so the rate of bacterial growth significantly decreased. Moreover, the results showed that the antibacterial effect of extracts on the growth of bacteria examined in this study is better than the essential oils studied and plant extracts in visit one or two weeks at the same time better than essential oils and show better appropriate action. According to the results of this study, essential oils and extracts of Pimpinella anisum and Artemisia dracunculus can be introduced as an appropriate alternative to commonly used Chemical bactericides against these bacteria.